Cerebral palsy (CP) is a group of permanent disorders including the development of movement and posture, in this study, we described an accurate quantification method for detecting mtDNA copy number on the basis of ddPCR technology.
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As a method that enables absolute quantification of nucleic acids, digital PCR (dPCR) has been proposed for non-invasive detection of fetal aneuploidy since 2007.
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Currently, various techniques have been applied to screen deafness-associated mutations in China. ddPCR is a new technology that enables absolute quantification of nucleic acids with high analytical sensitivity and precision.
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Droplet microfluidics is becoming an enabling technology for synthesizing microscale particles and an effective real-time method is essential to monitor the variations in a dynamic droplet generation process.
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Highly-sensitive and contamination-free droplet digital PCR (ddPCR) is an enabling technology and widely needed for accurate quantification of nucleic acid in clinical applications. In this paper, a novel droplet reader was developed.
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