CRISPR/Cas13‑assisted hepatitis B virus covalently closed circular DNA detection

In this paper, Professor Feng Ren used the digital PCR product from TargetingOne as a reference standard for assay sensitivity and positive detection rate, which can also test the effectiveness.

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Development of a Droplet Digital Polymerase Chain Reaction for Sensitive Detection of Pneumocystis jirovecii in Respiratory Tract Specimens

Droplet digital PCR (ddPCR), as a new and accurate quantitative technique, is an absolute quantitative method for the detection of genes (10, 11), especially for trace specimens (12–14).

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Interlaboratory assessment of quantification of SARS-CoV-2 RNA by reverse transcription digital PCR

For the analytical method, nine laboratories employed one-step RT-dPCR approach using kits corresponding to the spe-cific dPCR platforms. One laboratory employed a two-step RT-dPCR approach, where (cDNA) was synthesized in a separate reaction tube.

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Single-dose immunization with a chimpanzee adenovirus-based vaccine induces sustained and protective immunity against SARS-CoV-2 infection. Front. in Immun

Single-dose intranasal administration of this vaccine completely protected human DPP4 knock-in mice from lethal MERS-CoV challenge. Passive transfer of immune sera also conferred a survival advantage in lethal challenge mouse models (32).

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A SARS-CoVID-2 reference standard quantified by multiple digital PCR platforms for quality assessment of molecular tests

To determine the LoDs of diagnostic assays, various reference materials such as synthetic RNA transcripts, extracted genomic RNA, armored RNA, or inactivated virus particles with known concentrations or titers are commonly used.

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